Lab Blab #7

Lab Blab #7
The next day Jay and I came in to find that Dr. Bill had repeatedly exhausted the Fumigation box with fresh air to draw all the chloroform gas from the samples and the exhaust hood. We lifted the glass, arranged all in complete order for weighing. Now we would incorporate our control group into the picture. One-half of the 68 samples had been fumigated, and one-half was held for comparison without the lysing of the cells to release the organic Carbon. Everything we did from now on must be performed exactly the same, on both sets of samples. We weighed out small amounts of each sample, arranged them in the hood, and began adding reagents that turned our samples a spectrum of colors from aquamarine, to deep green, and brilliant shades of orange. A series of glucose-based standards were prepared, ranging from 0, 100 and up to 800 ul/g of glucose in solution. The colors and optical density would be indicators of the presence and concentration of organic Carbon. Now we have to perform careful extractions from our samples. Stay tuned for more…